Hotspot

psaC-rps7

SSC · 54842-56048 · 1207 bp

Back to Danxiaorchis

Pi 0.00783

MarkerSeek score 56.00

Primer available yes

Label psaC-rps7

Feature Metadata

label_name: psaC-rps7
feature_type: igs
region: SSC
length_bp: 1207
variable_sites: 8
pi: 0.00783
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-rps7_p1	ACATCCGTCCCAAGGTATCA	ATGGCAGAGGCAAATAGAGC	50.0	50.0	58.1	58.0	1185-1348 bp	1.0	83.3
psaC-rps7_p2	TTCCGATACATCTGTGGGGT	ATGGCAGAGGCAAATAGAGC	50.0	50.0	58.1	58.0	1164-1327 bp	1.0	83.3
psaC-rps7_p3	ACATCCGTCCCAAGGTATCA	GGCAGAGGCAAATAGAGCTT	50.0	50.0	58.1	58.0	1183-1346 bp	1.0	83.1
psaC-rps7_p4	TTCCGATACATCTGTGGGGT	GGCAGAGGCAAATAGAGCTT	50.0	50.0	58.1	58.0	1162-1325 bp	1.0	83.1
psaC-rps7_p5	CGGACACATTGGGTACATCC	ATGGCAGAGGCAAATAGAGC	55.0	50.0	58.3	58.0	1139-1302 bp	1.0	81.7