Hotspot

rpl32-trnL-UAG

SSC · 106971-107496 · 526 bp

Back to Danthoniopsis

Pi 0.0393

MarkerSeek score 68.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 526
variable_sites: 20
pi: 0.0393
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGAAGGATTGATTTGCGGCA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.2	57.9	805-815 bp	1.0	81.7
rpl32-trnL-UAG_p2	AGAAGGATTGATTTGCGGCA	GATCCGCACAAAAGAGTTGC	45.0	50.0	58.2	58.0	1384-1394 bp	1.0	81.0
rpl32-trnL-UAG_p3	AGAAGGATTGATTTGCGGCA	TGATGAATCGCGTAATCCCC	45.0	50.0	58.2	57.8	1046-1056 bp	1.0	80.4
rpl32-trnL-UAG_p4	AGAAGGATTGATTTGCGGCA	AATGATTAGAAGAGCGGCGG	45.0	50.0	58.2	58.1	1412-1422 bp	1.0	80.2
rpl32-trnL-UAG_p5	AGAAGGATTGATTTGCGGCA	TGTCCTGAAGAAACCCAACG	45.0	50.0	58.2	58.0	1127 bp	0.5	58.4