Hotspot

trnP-UGG-psaJ

LSC · 64801-65127 · 327 bp

Back to Danthoniopsis

Pi 0.0306

MarkerSeek score 62.10

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 327
variable_sites: 10
pi: 0.0306
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	AAACCTGCTAAAGCCCCAAA	45.0	45.0	57.9	57.9	437 bp	1.0	80.2
trnP-UGG-psaJ_p2	CAAAACAAACGCGCTACCAA	AAACCTGCTAAAGCCCCAAA	45.0	45.0	58.2	57.9	438 bp	1.0	79.7
trnP-UGG-psaJ_p3	TTGTACCCAAAACAAACGCG	AAACCTGCTAAAGCCCCAAA	45.0	45.0	57.8	57.9	445 bp	1.0	79.5
trnP-UGG-psaJ_p4	TACCCAAAACAAACGCGCTA	AAACCTGCTAAAGCCCCAAA	45.0	45.0	58.4	57.9	442 bp	1.0	77.8
trnP-UGG-psaJ_p5	AAAACAAACGCGCTACCAAG	CTGCTAAAGCCCCAAACCAT	45.0	50.0	57.9	58.4	433 bp	1.0	77.4