Hotspot

rbcL-psaI

LSC · 56770-57698 · 929 bp

Back to Danthoniopsis

Pi 0.0132

MarkerSeek score 53.90

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 929
variable_sites: 12
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GGCTTCAGGGGGTATTCATG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1281-1300 bp	1.0	81.8
rbcL-psaI_p2	GGCTTCAGGGGGTATTCATG	CTCGCTATACTCGGTTACGC	55.0	55.0	58.0	58.1	1499-1517 bp	1.0	81.4
rbcL-psaI_p3	AAGCCGGTAGATACCATCGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1023 bp	0.5	59.7
rbcL-psaI_p4	AAGCCGGTAGATACCATCGA	CTCGCTATACTCGGTTACGC	50.0	55.0	58.0	58.1	1240 bp	0.5	59.5
rbcL-psaI_p5	AAGCCGGTAGATACCATCGA	CCAAGATAACTGGAAGCCCC	50.0	55.0	58.0	57.9	1549 bp	0.5	59.5