Hotspot

trnF-GAA-ndhJ

LSC · 48455-49025 · 571 bp

Back to Danthoniopsis

Pi 0.00899

MarkerSeek score 63.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 571
variable_sites: 5
pi: 0.00899
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCGGAAAGTTGGATAGGCTG	50.0	55.0	58.1	58.3	672-683 bp	1.0	79.3
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CGGAAAGTTGGATAGGCTGG	50.0	55.0	58.1	58.3	671-682 bp	1.0	79.3
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATGCCGGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	675-686 bp	1.0	79.1
trnF-GAA-ndhJ_p4	ATGCACAAGAAAGGGTCAGG	CCGGAAAGTTGGATAGGCTG	50.0	55.0	58.1	58.3	725-736 bp	1.0	78.9
trnF-GAA-ndhJ_p5	ATGCACAAGAAAGGGTCAGG	CGGAAAGTTGGATAGGCTGG	50.0	55.0	58.1	58.3	724-735 bp	1.0	78.9