Hotspot

trnT-UGU-trnL-UAA

LSC · 46619-47409 · 791 bp

Back to Danthoniopsis

Pi 0.0165

MarkerSeek score 62.60

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 791
variable_sites: 13
pi: 0.0165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.0	58.0	892-898 bp	1.0	81.3
trnT-UGU-trnL-UAA_p2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.1	58.0	884-890 bp	1.0	80.9
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	55.0	50.0	57.9	58.0	862-868 bp	1.0	80.4
trnT-UGU-trnL-UAA_p4	ATTTGAACCGATGACCCTCG	GCGTCTACCTATTTCGCCAT	50.0	50.0	58.0	57.8	877-883 bp	1.0	80.1
trnT-UGU-trnL-UAA_p5	CGATGACCCTCGCATTACAA	GCGTCTACCTATTTCGCCAT	50.0	50.0	58.1	57.8	869-875 bp	1.0	79.8