Hotspot

trnS-GGA-rps4

LSC · 45336-45623 · 288 bp

Back to Danthoniopsis

Pi 0.0214

MarkerSeek score 67.40

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 288
variable_sites: 6
pi: 0.0214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	TTATGGAGGAGAGATGGCCG	AGATAGGAAGTGGGTCGGTC	55.0	55.0	58.7	58.2	444-452 bp	1.0	75.5
trnS-GGA-rps4_p2	TTATGGAGGAGAGATGGCCG	ATAGGAAGTGGGTCGGTCTC	55.0	55.0	58.7	58.2	442-450 bp	1.0	75.5
trnS-GGA-rps4_p3	TTATGGAGGAGAGATGGCCG	GATAGGAAGTGGGTCGGTCT	55.0	55.0	58.7	58.2	443-451 bp	1.0	75.5
trnS-GGA-rps4_p4	TTATGGAGGAGAGATGGCCG	GAAGTGGGTCGGTCTCAAAA	55.0	50.0	58.7	57.8	438-446 bp	1.0	75.3
trnS-GGA-rps4_p5	TTCGAATCCCTCTCTTTCCG	ATAGGAAGTGGGTCGGTCTC	50.0	55.0	57.0	58.2	368-376 bp	1.0	73.5