Hotspot

psaA-ycf3

LSC · 42015-42668 · 654 bp

Back to Danthoniopsis

Pi 0.00922

MarkerSeek score 64.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 654
variable_sites: 6
pi: 0.00922
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTCTGGCCCATTCCTCAAAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.1	58.0	812-815 bp	1.0	80.2
psaA-ycf3_p2	GTCTGGCCCATTCCTCAAAA	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.1	58.1	808-811 bp	1.0	79.8
psaA-ycf3_p3	CTGGCCCATTCCTCAAAAGA	TATAGCGCTTACTCCGGGAA	50.0	50.0	57.8	58.0	810-813 bp	1.0	79.0
psaA-ycf3_p4	TCTGGCCCATTCCTCAAAAG	TATAGCGCTTACTCCGGGAA	50.0	50.0	57.8	58.0	811-814 bp	1.0	79.0
psaA-ycf3_p5	CTGGCCCATTCCTCAAAAGA	GCGCTTACTCCGGGAAATTA	50.0	50.0	57.8	58.1	806-809 bp	1.0	78.7