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psbA-trnK-UUU

LSC · 1150-1377 · 228 bp

Back to Danthoniopsis

Pi 0.0263

MarkerSeek score 66.90

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 228
variable_sites: 6
pi: 0.0263
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TAGGCTTGTACTTTCGCGTC	AAATGCAAGCACGATTTGGG	50.0	45.0	58.0	57.9	373 bp	1.0	80.5
psbA-trnK-UUU_p2	GGGATCATCAACACACCGAA	AAATGCAAGCACGATTTGGG	50.0	45.0	57.9	57.9	450 bp	1.0	79.5
psbA-trnK-UUU_p3	ATCAACACACCGAACCATCC	AAATGCAAGCACGATTTGGG	50.0	45.0	58.2	57.9	444 bp	1.0	79.0
psbA-trnK-UUU_p4	TAGGCTTGTACTTTCGCGTC	CGATTTGGGGAGGGGTTTTT	50.0	50.0	58.0	58.4	362 bp	1.0	78.3
psbA-trnK-UUU_p5	GCTTGTACTTTCGCGTCTCT	AAATGCAAGCACGATTTGGG	50.0	45.0	58.3	57.9	370 bp	1.0	78.2