Hotspot

rps16

LSC · 4514-5598 · 1085 bp

Back to Danthonia

Pi 0.00492

MarkerSeek score 62.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1085
variable_sites: 8
pi: 0.00492
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GGTACTAGTGACCCTCTCCC	TCTCGATACCACTTTGCAATGA	60.0	40.9	57.9	57.8	1168-1191 bp	1.0	63.9
rps16_p2	AGTGACCCTCTCCCACTTTT	TCTCGATACCACTTTGCAATGA	50.0	40.9	58.2	57.8	1162-1185 bp	1.0	63.1
rps16_p3	CTAGTGACCCTCTCCCACTT	TCTCGATACCACTTTGCAATGA	55.0	40.9	57.8	57.8	1164-1187 bp	1.0	62.6
rps16_p4	GGTACTAGTGACCCTCTCCC	CTCGATACCACTTTGCAATGA	60.0	42.9	57.9	56.3	1167-1190 bp	1.0	60.3
rps16_p5	GGTACTAGTGACCCTCTCCC	TCTCGATACCACTTTGCAATG	60.0	42.9	57.9	56.3	1168-1191 bp	1.0	60.3