Hotspot

rps11-rpl36

LSC · 75103-75266 · 164 bp

Back to Danthonia

Pi 0.0314

MarkerSeek score 63.90

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 164
variable_sites: 7
pi: 0.0314
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TGCGTAAACTAAAACGGGCA	CCGAAGCATAAACAAAGACAGG	45.0	45.5	58.4	57.9	268-273 bp	1.0	63.6
rps11-rpl36_p2	TGCGTAAACTAAAACGGGCA	CGAAGCATAAACAAAGACAGGG	45.0	45.5	58.4	57.9	267-272 bp	1.0	63.6
rps11-rpl36_p3	TGCGTAAACTAAAACGGGCA	ATTTGTTCCAATCCGAAGCA	45.0	40.0	58.4	55.9	280-285 bp	1.0	63.5
rps11-rpl36_p4	TGCGTAAACTAAAACGGGCA	TTTGTTCCAATCCGAAGCAT	45.0	40.0	58.4	55.9	279-284 bp	1.0	63.5
rps11-rpl36_p5	TAAAACGGGCATTCCTACGC	TTTGTTCCAATCCGAAGCAT	50.0	40.0	58.6	55.9	270-275 bp	1.0	61.9