Hotspot

ycf3-trnS-GGA

LSC · 44161-44745 · 585 bp

Back to Danthonia

Pi 0.00456

MarkerSeek score 61.50

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 585
variable_sites: 4
pi: 0.00456
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TCTCCTGAGGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.1	58.2	767-768 bp	1.0	78.8
ycf3-trnS-GGA_p2	TCTCCTGAGGTTGTCGGAAT	GCAGTTCCAATGCTACACCT	50.0	50.0	58.1	58.2	721-722 bp	1.0	78.7
ycf3-trnS-GGA_p3	TTCTCCTGAGGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	768-769 bp	1.0	77.0
ycf3-trnS-GGA_p4	TTTCTCCTGAGGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	769-770 bp	1.0	77.0
ycf3-trnS-GGA_p5	TTCTCCTGAGGTTGTCGGAA	GCAGTTCCAATGCTACACCT	50.0	50.0	58.3	58.2	722-723 bp	1.0	76.9