Hotspot

trnG-GCC-trnfM-CAU

LSC · 12313-12657 · 345 bp

Back to Danthonia

Pi 0.00777

MarkerSeek score 50.20

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 345
variable_sites: 4
pi: 0.00777
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	GCGAGCGTAGTTCAATGGTA	TCTACAGAGCGGAGTAGAGC	50.0	55.0	58.1	58.0	496-519 bp	1.0	80.3
trnG-GCC-trnfM-CAU_p2	CGTAGCGAGCGTAGTTCAAT	TCTACAGAGCGGAGTAGAGC	50.0	55.0	58.2	58.0	500-523 bp	1.0	79.5
trnG-GCC-trnfM-CAU_p3	TAGCGAGCGTAGTTCAATGG	TCTACAGAGCGGAGTAGAGC	50.0	55.0	57.8	58.0	498-521 bp	1.0	79.3
trnG-GCC-trnfM-CAU_p4	GCGAGCGTAGTTCAATGGTA	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.1	57.8	462-485 bp	1.0	78.9
trnG-GCC-trnfM-CAU_p5	GCGAGCGTAGTTCAATGGTA	CAGTTTGGTAGCTCACGAGG	50.0	55.0	58.1	58.3	477-500 bp	1.0	78.4