Hotspot

trnK-UUU-rps16

LSC · 3936-4513 · 578 bp

Back to Danthonia

Pi 0.00122

MarkerSeek score 63.50

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 578
variable_sites: 1
pi: 0.00122
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCACTTAAAAGCCGAG	GGTGCTCAACCTACAAGAACT	50.0	47.6	58.6	57.9	679-712 bp	1.0	70.4
trnK-UUU-rps16_p2	TAGTCGCACTTAAAAGCCGA	GGTGCTCAACCTACAAGAACT	45.0	47.6	57.3	57.9	680-713 bp	1.0	69.1
trnK-UUU-rps16_p3	TCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAAGAACT	45.0	47.6	57.3	57.9	677-710 bp	1.0	69.1
trnK-UUU-rps16_p4	CGCACTTAAAAGCCGAGTAC	GGTGCTCAACCTACAAGAACT	50.0	47.6	57.2	57.9	676-709 bp	1.0	68.7
trnK-UUU-rps16_p5	GTCGCACTTAAAAGCCGAGT	GGTGCTCAACCTACAAGAACT	50.0	47.6	58.9	57.9	678-711 bp	1.0	68.1