Hotspot

ndhH

SSC · 123887-125068 · 1182 bp

Back to Dalenia

Pi 0.00169

MarkerSeek score 59.70

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 2
pi: 0.00169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	AAAGCATCCCTATGATCCCA	ACTTCTTTTCGTTTTAAGCAGTTCA	45.0	32.0	55.9	58.2	1325 bp	1.0	40.1
ndhH_p2	AAAGCATCCCTATGATCCCA	TCTTTTCGTTTTAAGCAGTTCA	45.0	31.8	55.9	54.8	1322 bp	1.0	40.1
ndhH_p3	AAAGCATCCCTATGATCCCA	ACTTCTTTTCGTTTTAAGCAGT	45.0	31.8	55.9	54.8	1325 bp	1.0	40.1
ndhH_p4	AAAGCATCCCTATGATCCCA	CTTCTTTTCGTTTTAAGCAGTTCA	45.0	33.3	55.9	56.7	1324 bp	1.0	40.1
ndhH_p5	AAAGCATCCCTATGATCCCA	ACTTCTTTTCGTTTTAAGCAGTTC	45.0	33.3	55.9	56.7	1325 bp	1.0	40.1