Hotspot

trnC-GCA-ndhJ

LSC · 49811-50661 · 851 bp

Back to Dalenia

Pi 0.00239

MarkerSeek score 61.70

Primer available yes

Label trnC-GCA-ndhJ

Feature Metadata

label_name: trnC-GCA-ndhJ
feature_type: igs
region: LSC
length_bp: 851
variable_sites: 2
pi: 0.00239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ACTCATCCACGACTGAAACG	55.0	50.0	58.0	57.9	1015-1029 bp	1.0	80.7
trnC-GCA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	CTCATCCACGACTGAAACGT	55.0	50.0	58.0	57.9	1014-1028 bp	1.0	80.7
trnC-GCA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	979-993 bp	1.0	80.6
trnC-GCA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ACTCATCCACGACTGAAACG	50.0	50.0	58.1	57.9	979-993 bp	1.0	80.4
trnC-GCA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	CTCATCCACGACTGAAACGT	50.0	50.0	58.1	57.9	978-992 bp	1.0	80.4