Hotspot

rps4-trnT-UGU

LSC · 47553-47954 · 402 bp

Back to Dalenia

Pi 0.0124

MarkerSeek score 56.50

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 402
variable_sites: 5
pi: 0.0124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAGACCTGGTAAAGCCCCAA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	528 bp	1.0	79.3
rps4-trnT-UGU_p2	TTAGACCTGGTAAAGCCCCA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	529 bp	1.0	79.3
rps4-trnT-UGU_p3	AAAATCGCTTCCGGCTCTAG	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	561 bp	1.0	79.2
rps4-trnT-UGU_p4	TGAAACGAGGCCCCCTATAA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	493 bp	1.0	78.8
rps4-trnT-UGU_p5	TTGAAACGAGGCCCCCTATA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	494 bp	1.0	78.8