Hotspot

psaC

SSC · 123156-123401 · 246 bp

Pi 0.511

MarkerSeek score 71.00

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 91
pi: 0.511
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	CTGAAGCAGCTATCGGACTG	TCAGAAAAACCCGTGCTCAA	55.0	45.0	58.1	58.0	717 bp	0.5	58.4
psaC_p2	CTGAAGCAGCTATCGGACTG	GAACTTCCCGCAGTGTACAA	55.0	50.0	58.1	58.1	1264 bp	0.5	57.8
psaC_p3	CTGAAGCAGCTATCGGACTG	CGCCACAGGATACGATTGAT	55.0	50.0	58.1	57.8	1364 bp	0.5	57.6
psaC_p4	CTGAAGCAGCTATCGGACTG	AACCCCGTCAGTAAAATGGG	55.0	50.0	58.1	57.8	1024 bp	0.5	57.1
psaC_p5	CTGAAGCAGCTATCGGACTG	CCCGGCTATTGGCAAAACTA	55.0	50.0	58.1	58.2	791 bp	0.5	56.9