Hotspot

ndhH

SSC · 117303-118484 · 1182 bp

Pi 0.495

MarkerSeek score 83.10

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 543
pi: 0.495
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	AAAACCACGGATCGGTTGAA	CTAGGAAAGCGGCATAGCAA	45.0	50.0	58.0	58.0	2113 bp	0.5	59.4
ndhH_p2	AAAACCACGGATCGGTTGAA	CGTTGTTGTATCCCTGCAGA	45.0	50.0	58.0	57.9	1687 bp	0.5	59.0
ndhH_p3	AAAACCACGGATCGGTTGAA	AAGCGGCATAGCAATAGCAT	45.0	45.0	58.0	58.1	2107 bp	0.5	59.0
ndhH_p4	AAAACCACGGATCGGTTGAA	GCTTGAAGAACTCCTCCAGG	45.0	55.0	58.0	57.9	1729 bp	0.5	58.9
ndhH_p5	AGAAGAAAACCACGGATCGG	CTAGGAAAGCGGCATAGCAA	50.0	50.0	57.9	58.0	2118 bp	0.5	58.9