Hotspot

ndhG

SSC · 121825-122355 · 531 bp

Back to Dalea

Pi 0.416
MarkerSeek score 78.80
Primer available yes
Label ndhG

Feature Metadata

label_name
ndhG
feature_type
gene
region
SSC
length_bp
531
variable_sites
184
pi
0.416
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG_p1 GAGGGGAATGACATTGGTCC CAGTCCGATAGCTGCTTCAG 55.0 55.0 57.9 58.1 1133 bp 0.5 58.3
ndhG_p2 AATCAAATTGCTTTGGGCCG CAGTCCGATAGCTGCTTCAG 45.0 55.0 57.9 58.1 1519 bp 0.5 58.0
ndhG_p3 GAGGGGAATGACATTGGTCC TTTCGGCTCGTGATCAATCC 55.0 50.0 57.9 58.3 976 bp 0.5 56.7
ndhG_p4 TTTGGGCCGTTTACCAATGT CAGTCCGATAGCTGCTTCAG 45.0 55.0 58.3 58.1 1508 bp 0.5 56.4
ndhG_p5 AATCAAATTGCTTTGGGCCG TTTCGGCTCGTGATCAATCC 45.0 50.0 57.9 58.3 1362 bp 0.5 56.4