Hotspot

ndhA

SSC · 118486-120786 · 2301 bp

Pi 0.449

MarkerSeek score 78.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2301
variable_sites: 838
pi: 0.449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AGAGTTGAAGCCCCAAAAGG	CTCGGACTGTTTGTTGACCA	50.0	50.0	58.0	58.1	2631 bp	0.5	59.6
ndhA_p2	TTCGTCCACCGGGTTTTATC	CTCGGACTGTTTGTTGACCA	50.0	50.0	57.9	58.1	2555 bp	0.5	58.8
ndhA_p3	TGGGGTTTATCAGGACCGAT	CTCGGACTGTTTGTTGACCA	50.0	50.0	58.1	58.1	2967 bp	0.5	58.7
ndhA_p4	TCCGACTTTTGAATTGCCGA	CTCGGACTGTTTGTTGACCA	45.0	50.0	58.1	58.1	2668 bp	0.5	58.7
ndhA_p5	TTCGTCCACCGGGTTTTATC	CGGCCCAAAGCAATTTGATT	50.0	45.0	57.9	57.9	2934 bp	0.5	58.4