Hotspot

ccsA

SSC · 125284-126255 · 972 bp

Back to Dalea

Pi 0.484
MarkerSeek score 81.40
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
972
variable_sites
410
pi
0.484
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 TTATCTCGACGAAATGGGCG GTGAAATCGGTAGACACGCT 50.0 50.0 58.2 58.0 1830 bp 0.5 58.7
ccsA_p2 TTATCTCGACGAAATGGGCG TGAAATCGGTAGACACGCTG 50.0 50.0 58.2 58.0 1829 bp 0.5 58.7
ccsA_p3 GATGGCTTCTCTTGCATTGC GTGAAATCGGTAGACACGCT 50.0 50.0 57.8 58.0 1761 bp 0.5 58.3
ccsA_p4 GATGGCTTCTCTTGCATTGC TGAAATCGGTAGACACGCTG 50.0 50.0 57.8 58.0 1760 bp 0.5 58.3
ccsA_p5 CGGGGCCGAGAGAATTATTT GTGAAATCGGTAGACACGCT 50.0 50.0 57.7 58.0 1537 bp 0.5 57.9