Hotspot

trnL-UAG-rpl32

SSC · 126431-127103 · 673 bp

Back to Dalea

Pi 0.427
MarkerSeek score 80.90
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
673
variable_sites
246
pi
0.427
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.86
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC AAAAGCGCACTTCCGTATCA 50.0 45.0 58.0 58.2 836 bp 0.5 58.4
trnL-UAG-rpl32_p2 AGCGTGTCTACCGATTTCAC AAAGCGCACTTCCGTATCAA 50.0 45.0 58.0 58.2 835 bp 0.5 58.4
trnL-UAG-rpl32_p3 AGCGTGTCTACCGATTTCAC AAGCGCACTTCCGTATCAAA 50.0 45.0 58.0 58.2 834 bp 0.5 58.4
trnL-UAG-rpl32_p4 AGCGTGTCTACCGATTTCAC AGCGCACTTCCGTATCAAAA 50.0 45.0 58.0 58.2 833 bp 0.5 58.4
trnL-UAG-rpl32_p5 CAGCGTGTCTACCGATTTCA AGCGCACTTCCGTATCAAAA 50.0 45.0 58.0 58.2 834 bp 0.5 58.4