Hotspot

rps15-ndhH

SSC · 117196-117302 · 107 bp

Back to Dalea

Pi 0.455
MarkerSeek score 77.20
Primer available yes
Label rps15-ndhH

Feature Metadata

label_name
rps15-ndhH
feature_type
igs
region
SSC
length_bp
107
variable_sites
46
pi
0.455
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ndhH_p1 AAAACCACGGATCGGTTGAA AACACCATGCATTGATGGGT 45.0 45.0 58.0 58.1 397 bp 0.5 59.3
rps15-ndhH_p2 AAAACCACGGATCGGTTGAA TGCCATAAAAGGTCCCAACC 45.0 50.0 58.0 58.1 691 bp 0.5 59.2
rps15-ndhH_p3 AAAACCACGGATCGGTTGAA ATTTCCCAACAGTTCCGGTC 45.0 50.0 58.0 58.1 595 bp 0.5 59.0
rps15-ndhH_p4 AAAACCACGGATCGGTTGAA ATCGGTCCTGATAAACCCCA 45.0 50.0 58.0 58.1 990 bp 0.5 58.9
rps15-ndhH_p5 AGAAGAAAACCACGGATCGG AACACCATGCATTGATGGGT 50.0 45.0 57.9 58.1 402 bp 0.5 58.8