Hotspot

ycf1

SSC · 125668-130974 · 5307 bp

Back to Dalbergia

Pi 0.0283

MarkerSeek score 62.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5307
variable_sites: 962
pi: 0.0283
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCAGGCCTCTCCTATTTCT	GAAGCGTCCGTTGCGATATA	55.0	50.0	58.2	58.2	1289-1331 bp	0.975	86.7
ycf1_p2	TCCCAGGCCTCTCCTATTTC	GAAGCGTCCGTTGCGATATA	55.0	50.0	58.2	58.2	1290-1332 bp	0.975	86.7
ycf1_p3	CGCCTTTGAGCACGTATAGA	GAAGCGTCCGTTGCGATATA	50.0	50.0	57.8	58.2	1044-1062 bp	0.975	84.8
ycf1_p4	TCCCCCTCCTCACTTTCTTT	GAAGCGTCCGTTGCGATATA	50.0	50.0	57.9	58.2	1254-1272 bp	0.85	80.2
ycf1_p5	TCCCCCTCCTCACTTTCTTT	AAGCGTCCGTTGCGATATAT	50.0	45.0	57.9	57.6	1253-1271 bp	0.875	79.6