Hotspot

rps16

LSC · 56064-57232 · 1169 bp

Back to Dalbergia

Pi 0.0211

MarkerSeek score 73.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1169
variable_sites: 163
pi: 0.0211
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACCGGATCCAAACTAAAGGA	AAGTCATCCACACCCTCCTT	45.0	50.0	56.1	58.3	1201-1253 bp	0.95	73.1
rps16_p2	ACCGGATCCAAACTAAAGGA	AGTCATCCACACCCTCCTTT	45.0	50.0	56.1	58.3	1200-1252 bp	0.95	73.1
rps16_p3	ACCGGATCCAAACTAAAGGA	GTCATCCACACCCTCCTTTT	45.0	50.0	56.1	57.4	1199-1251 bp	0.975	71.4
rps16_p4	ACCGGATCCAAACTAAAGGAA	AAGTCATCCACACCCTCCTT	42.9	50.0	56.9	58.3	1201-1253 bp	0.95	71.3
rps16_p5	ACCGGATCCAAACTAAAGGAA	AGTCATCCACACCCTCCTTT	42.9	50.0	56.9	58.3	1200-1252 bp	0.95	71.3