Hotspot

ndhG-ndhI

SSC · 120028-120781 · 754 bp

Back to Dalbergia

Pi 0.0724

MarkerSeek score 75.30

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 754
variable_sites: 265
pi: 0.0724
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CAACCAAGAACCAGTCCCAA	GCGGGAACTGTGTTGAGTAT	50.0	50.0	57.9	57.9	862-1180 bp	1.0	90.7
ndhG-ndhI_p2	CAACCAAGAACCAGTCCCAA	TGGTCAACAAACAGTCCGAG	50.0	50.0	57.9	58.1	1149-1467 bp	0.975	90.1
ndhG-ndhI_p3	CCCCAACGGTCAAAAGATGA	GCGGGAACTGTGTTGAGTAT	50.0	50.0	58.1	57.9	1017-1335 bp	0.9	86.5
ndhG-ndhI_p4	CCCCAACGGTCAAAAGATGA	TGGTCAACAAACAGTCCGAG	50.0	50.0	58.1	58.1	1304-1622 bp	0.875	85.8
ndhG-ndhI_p5	TTGCACCTATCAAAGCAGCT	TGGTCAACAAACAGTCCGAG	45.0	50.0	57.8	58.1	1569-1843 bp	0.85	83.9