Hotspot

rps8-rpl14

LSC · 81745-82088 · 344 bp

Back to Dalbergia

Pi 0.0404

MarkerSeek score 76.90

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 344
variable_sites: 71
pi: 0.0404
unique_haplotype_count: 33
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TTATGTCAGCGATGGTGTCC	AGAATTTTTGGCGCAATCCC	50.0	45.0	57.7	57.6	393-494 bp	1.0	86.4
rps8-rpl14_p2	GTCAGCGATGGTGTCCTTAC	AGAATTTTTGGCGCAATCCC	55.0	45.0	58.4	57.6	389-490 bp	1.0	86.1
rps8-rpl14_p3	TATGTCAGCGATGGTGTCCT	AGAATTTTTGGCGCAATCCC	50.0	45.0	58.5	57.6	392-493 bp	1.0	84.8
rps8-rpl14_p4	TTATGTCAGCGATGGTGTCC	GAATTTTTGGCGCAATCCCC	50.0	50.0	57.7	58.6	392-493 bp	0.925	81.5
rps8-rpl14_p5	GTCAGCGATGGTGTCCTTAC	GAATTTTTGGCGCAATCCCC	55.0	50.0	58.4	58.6	388-489 bp	0.925	81.1