Hotspot

trnP-UGG-psaJ

LSC · 66824-67546 · 723 bp

Back to Dalbergia

Pi 0.0409

MarkerSeek score 75.10

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 723
variable_sites: 176
pi: 0.0409
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	CGGTCTCAATTGATCCCCTG	TTTACCCTTGGCCATGAACC	55.0	50.0	58.0	58.1	1460-1544 bp	0.925	88.1
trnP-UGG-psaJ_p2	CGGTCTCAATTGATCCCCTG	CTTGGCCATGAACCTCCTTT	55.0	50.0	58.0	58.1	1454-1538 bp	0.925	88.1
trnP-UGG-psaJ_p3	TGATCCCCTGTTACTGCTCA	TCACCGAGTCCAAATACCCT	50.0	50.0	58.0	58.1	1121-1175 bp	0.45	68.3
trnP-UGG-psaJ_p4	GCGTTCTACCGAACTGAACT	TCACCGAGTCCAAATACCCT	50.0	50.0	57.9	58.1	1250-1305 bp	0.45	68.1
trnP-UGG-psaJ_p5	CGGTCTCAATTGATCCCCTG	TCACCGAGTCCAAATACCCT	55.0	50.0	58.0	58.1	1131-1185 bp	0.375	65.2