Hotspot

psbD-trnT-GGU

LSC · 28511-29783 · 1273 bp

Back to Dalbergia

Pi 0.0251

MarkerSeek score 73.10

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1273
variable_sites: 207
pi: 0.0251
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	CCCACAAAAACGAAACGGTC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.2	58.1	1221-1461 bp	1.0	90.1
psbD-trnT-GGU_p2	CACAAAAACGAAACGGTCCC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.2	58.1	1219-1459 bp	0.95	88.1
psbD-trnT-GGU_p3	CCACAAAAACGAAACGGTCC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.2	58.1	1220-1460 bp	0.95	88.1
psbD-trnT-GGU_p4	CACAAAAACGAAACGGTCCC	TCAGTGGTAGAGTAACGCCA	50.0	50.0	58.2	58.1	1222-1462 bp	0.95	88.0
psbD-trnT-GGU_p5	CCACAAAAACGAAACGGTCC	TCAGTGGTAGAGTAACGCCA	50.0	50.0	58.2	58.1	1223-1463 bp	0.95	88.0