Hotspot

ndhA

SSC · 118413-120814 · 2402 bp

Back to Dahlstedtia

Pi 0.0243

MarkerSeek score 57.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2402
variable_sites: 58
pi: 0.0243
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CTCGGACTGTTTGTTGACCA	TGTAAGAGTTGAGGCTCCCA	50.0	50.0	58.1	58.0	2729-2740 bp	1.0	81.7
ndhA_p2	TTGACCAATGTACCTCGCAG	TGTAAGAGTTGAGGCTCCCA	50.0	50.0	57.9	58.0	2749-2760 bp	1.0	81.3
ndhA_p3	CTCGGACTGTTTGTTGACCA	TTCGTCCACCGGGTTTTATC	50.0	50.0	58.1	57.9	2649-2660 bp	1.0	81.2
ndhA_p4	CTCGGACTGTTTGTTGACCA	TCCGACTTTTGAATTGCCGA	50.0	45.0	58.1	58.1	2762-2773 bp	1.0	80.9
ndhA_p5	TTGACCAATGTACCTCGCAG	TTCGTCCACCGGGTTTTATC	50.0	50.0	57.9	57.9	2669-2680 bp	1.0	80.8