Hotspot

ndhF-rpl32

SSC · 111366-112085 · 720 bp

Back to Dahlstedtia

Pi 0.0536

MarkerSeek score 79.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 720
variable_sites: 37
pi: 0.0536
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGAATCCACGAAGATTGATGTGT	TGATATAGATGTGCGCTTTT	39.1	35.0	58.2	52.8	756-785 bp	1.0	47.0
ndhF-rpl32_p2	GAATCCACGAAGATTGATGTGT	TGATATAGATGTGCGCTTTT	40.9	35.0	56.8	52.8	755-784 bp	1.0	47.0
ndhF-rpl32_p3	AGAATCCACGAAGATTGATGTGT	TTGATATAGATGTGCGCTTT	39.1	35.0	58.2	52.8	757-786 bp	1.0	46.9
ndhF-rpl32_p4	AGAATCCACGAAGATTGATGTGT	TTTGATATAGATGTGCGCTT	39.1	35.0	58.2	52.8	758-787 bp	1.0	46.9
ndhF-rpl32_p5	AGAATCCACGAAGATTGATGTGT	TTTTGATATAGATGTGCGCT	39.1	35.0	58.2	52.8	759-788 bp	1.0	46.9