Hotspot

rpl16-rps3

LSC · 82415-82503 · 89 bp

Back to Dahlstedtia

Pi 0.0562
MarkerSeek score 73.20
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
89
variable_sites
5
pi
0.0562
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 AAAGCAACGAGTCACACACT GGAGTATTGGGGATTAAAGTTTGG 45.0 41.7 58.0 57.5 160 bp 1.0 46.6
rpl16-rps3_p2 AAAGCAACGAGTCACACACT TGGAGTATTGGGGATTAAAGTTTG 45.0 37.5 58.0 56.9 161 bp 1.0 42.5
rpl16-rps3_p3 AAAGCAACGAGTCACACACT TGGAGTATTGGGGATTAAAGT 45.0 38.1 58.0 53.9 161 bp 1.0 42.5
rpl16-rps3_p4 AAAGCAACGAGTCACACACT CGAACTATTTATGGAGTATTGGGG 45.0 41.7 58.0 57.0 172 bp 1.0 42.3
rpl16-rps3_p5 AAAGCAACGAGTCACACACT CGAACTATTTATGGAGTATTGGGGA 45.0 40.0 58.0 58.2 172 bp 1.0 42.3