Hotspot

rpl20-rps12

LSC · 68146-68969 · 824 bp

Back to Dahlstedtia

Pi 0.0419
MarkerSeek score 72.40
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
824
variable_sites
34
pi
0.0419
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 AAGTCTTGAATGAGCTCCGC CGACGAGGGACATGTACAAG 50.0 55.0 58.3 58.1 938-944 bp 1.0 81.1
rpl20-rps12_p2 AAGTCTTGAATGAGCTCCGC AGGGACATGTACAAGGGTGT 50.0 50.0 58.3 58.3 933-939 bp 1.0 79.8
rpl20-rps12_p3 AAGTCTTGAATGAGCTCCGC GACGAGGGACATGTACAAGG 50.0 55.0 58.3 57.7 937-943 bp 1.0 79.5
rpl20-rps12_p4 AAGTCTTGAATGAGCTCCGC CAACGACGAGGGACATGTAC 50.0 55.0 58.3 58.4 941-947 bp 1.0 78.8
rpl20-rps12_p5 AAGTCTTGAATGAGCTCCGC GAGGGACATGTACAAGGGTG 50.0 55.0 58.3 57.6 934-940 bp 1.0 78.6