Hotspot

psaJ-rpl33

LSC · 66360-66847 · 488 bp

Back to Dahlstedtia

Pi 0.0249

MarkerSeek score 57.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 488
variable_sites: 12
pi: 0.0249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CATATCTTTCCGTGGCACCA	TTTTGTCCCCCGATCCAATC	50.0	50.0	58.0	57.9	1084-1097 bp	1.0	82.1
psaJ-rpl33_p2	CATATCTTTCCGTGGCACCA	TTGCAACAACAGGGACAGAA	50.0	45.0	58.0	57.9	772-777 bp	1.0	80.9
psaJ-rpl33_p3	CATATCTTTCCGTGGCACCA	TTTGCAACAACAGGGACAGA	50.0	45.0	58.0	57.9	773-778 bp	1.0	80.9
psaJ-rpl33_p4	ACATATCTTTCCGTGGCACC	TTGCAACAACAGGGACAGAA	50.0	45.0	58.0	57.9	773-778 bp	1.0	80.9
psaJ-rpl33_p5	ACATATCTTTCCGTGGCACC	TTTGCAACAACAGGGACAGA	50.0	45.0	58.0	57.9	774-779 bp	1.0	80.9