Hotspot

psbD-trnT-GGU

LSC · 28554-29341 · 788 bp

Back to Dahlstedtia

Pi 0.0325
MarkerSeek score 74.50
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
LSC
length_bp
788
variable_sites
24
pi
0.0325
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 CACAAAAACGAAACGGTCCC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 939-988 bp 1.0 84.1
psbD-trnT-GGU_p2 CCACAAAAACGAAACGGTCC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 940-989 bp 1.0 84.1
psbD-trnT-GGU_p3 CCCACAAAAACGAAACGGTC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 941-990 bp 1.0 84.1
psbD-trnT-GGU_p4 CACAAAAACGAAACGGTCCC TCAGTGGTAGAGTAACGCCA 50.0 50.0 58.2 58.1 942-991 bp 1.0 83.9
psbD-trnT-GGU_p5 CCACAAAAACGAAACGGTCC TCAGTGGTAGAGTAACGCCA 50.0 50.0 58.2 58.1 943-992 bp 1.0 83.9