Hotspot

trnG-GCC-psbZ

LSC · 24747-25188 · 442 bp

Back to Dahlstedtia

Pi 0.0399

MarkerSeek score 81.70

Primer available yes

Label trnG-GCC-psbZ

Feature Metadata

label_name: trnG-GCC-psbZ
feature_type: igs
region: LSC
length_bp: 442
variable_sites: 17
pi: 0.0399
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-psbZ_p1	CGTCTTCTCCTTGGCAAAGA	CTTCTCCTGATGGTTGGTCG	50.0	55.0	57.8	58.0	596-599 bp	1.0	82.9
trnG-GCC-psbZ_p2	CGTCTTCTCCTTGGCAAAGA	TTGCTTCTCCTGATGGTTGG	50.0	50.0	57.8	57.8	599-602 bp	1.0	81.4
trnG-GCC-psbZ_p3	CGTCTTCTCCTTGGCAAAGA	TCCTGATGGTTGGTCGAGTA	50.0	50.0	57.8	57.8	592-595 bp	1.0	81.3
trnG-GCC-psbZ_p4	CGTCTTCTCCTTGGCAAAGA	GCTTCTCCTGATGGTTGGTC	50.0	55.0	57.8	58.3	597-600 bp	1.0	81.0
trnG-GCC-psbZ_p5	CGTCTTCTCCTTGGCAAAGA	TTCTCCTGATGGTTGGTCGA	50.0	50.0	57.8	58.4	595-598 bp	1.0	80.2