Hotspot

ycf3-psaA

LSC · 18522-19442 · 921 bp

Back to Dahlstedtia

Pi 0.0347

MarkerSeek score 75.00

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 921
variable_sites: 31
pi: 0.0347
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	AGCCCTTACCCCTGGTAATT	TTTCACTTCTGGTTCCGGTG	50.0	50.0	58.0	58.0	1021-1052 bp	1.0	84.2
ycf3-psaA_p2	AGCTATAGCCCTTACCCCTG	TTTCACTTCTGGTTCCGGTG	55.0	50.0	58.0	58.0	1027-1058 bp	1.0	84.0
ycf3-psaA_p3	TTGGTTGAAGATCACAGGGC	TTTCACTTCTGGTTCCGGTG	50.0	50.0	58.1	58.0	985-1016 bp	1.0	83.7
ycf3-psaA_p4	AGCCCTTACCCCTGGTAATT	TGCCCATTCCTCGAAAGAAG	50.0	50.0	58.0	57.9	1069-1100 bp	1.0	83.4
ycf3-psaA_p5	AGCCCTTACCCCTGGTAATT	GCCCATTCCTCGAAAGAAGT	50.0	50.0	58.0	57.9	1068-1099 bp	1.0	83.4