Hotspot

rpl32-trnL-UAG

SSC · 112167-112945 · 779 bp

Back to Dahlia

Pi 0.0118
MarkerSeek score 67.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
779
variable_sites
9
pi
0.0118
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAAGGAAAGGATATGGGGCG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.6 58.0 901-912 bp 1.0 79.0
rpl32-trnL-UAG_p2 AAAGGAAAGGATATGGGGCG CAGCGTGTCTACCGATTTCA 50.0 50.0 57.6 58.0 902-913 bp 1.0 79.0
rpl32-trnL-UAG_p3 CGGCGTTAAAAGCTTTGTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.6 58.0 883-894 bp 1.0 78.9
rpl32-trnL-UAG_p4 CGGCGTTAAAAGCTTTGTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.6 58.0 884-895 bp 1.0 78.9
rpl32-trnL-UAG_p5 AAAGGAAAGGATATGGGGCG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.6 57.9 911-922 bp 1.0 78.1