Hotspot

ndhF-rpl32

SSC · 111009-112001 · 993 bp

Back to Dahlia

Pi 0.00806
MarkerSeek score 63.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
993
variable_sites
8
pi
0.00806
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGTCGGAAAAAGAAG CGCCCCATATCCTTTCCTTT 50.0 50.0 58.0 57.6 1172-1176 bp 1.0 77.4
ndhF-rpl32_p2 TGCCGTCGGAAAAAGAAGAA CGCCCCATATCCTTTCCTTT 45.0 50.0 58.0 57.6 1170-1174 bp 1.0 77.3
ndhF-rpl32_p3 TTGCCGTCGGAAAAAGAAGA CGCCCCATATCCTTTCCTTT 45.0 50.0 58.0 57.6 1171-1175 bp 1.0 77.3
ndhF-rpl32_p4 GTTGCCGTCGGAAAAAGAAG TGACAAAGCTTTTAACGCCG 50.0 45.0 58.0 57.6 1190-1194 bp 1.0 77.1
ndhF-rpl32_p5 TGCCGTCGGAAAAAGAAGAA TGACAAAGCTTTTAACGCCG 45.0 45.0 58.0 57.6 1188-1192 bp 1.0 77.0