Hotspot

atpB-rbcL

LSC · 54028-54785 · 758 bp

Back to Dahlia

Pi 0.00792
MarkerSeek score 63.30
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
758
variable_sites
6
pi
0.00792
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CTGGCGGAAAAGCTACATCT CACCAGCTTTGAATCCAACAC 50.0 47.6 58.0 58.0 919-942 bp 1.0 72.1
atpB-rbcL_p2 CTGGCGGAAAAGCTACATCT ACCAGCTTTGAATCCAACACT 50.0 42.9 58.0 58.1 918-941 bp 1.0 71.9
atpB-rbcL_p3 GGCAACCCCAGAACTAGAAG CACCAGCTTTGAATCCAACAC 55.0 47.6 57.9 58.0 846-869 bp 1.0 71.5
atpB-rbcL_p4 GATACGCCCCAGGTTCTTTT CACCAGCTTTGAATCCAACAC 50.0 47.6 57.9 58.0 876-899 bp 1.0 71.4
atpB-rbcL_p5 GGCAACCCCAGAACTAGAAG ACCAGCTTTGAATCCAACACT 55.0 42.9 57.9 58.1 845-868 bp 1.0 71.2