Hotspot

ndhC-trnV-UAC

LSC · 49997-51009 · 1013 bp

Back to Dahlia

Pi 0.0129
MarkerSeek score 66.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1013
variable_sites
13
pi
0.0129
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGCTAATCGGAGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1156-1158 bp 1.0 76.9
ndhC-trnV-UAC_p2 CGGTCCTTTGCTAATCGGAG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.4 57.8 1158-1160 bp 1.0 76.0
ndhC-trnV-UAC_p3 CTCCGGTCCTTTGCTAATCG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.4 57.8 1161-1163 bp 1.0 76.0
ndhC-trnV-UAC_p4 GTCCTTTGCTAATCGGAGCT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.7 57.5 1155-1157 bp 1.0 74.2
ndhC-trnV-UAC_p5 GGTCCTTTGCTAATCGGAGC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.7 57.8 1157-1159 bp 1.0 73.9