Hotspot

psbZ-trnG-GCC

LSC · 35762-36090 · 329 bp

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Pi 0.0547
MarkerSeek score 82.40
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
329
variable_sites
18
pi
0.0547
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTGCTTCTCCTGATGGTTGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 486-495 bp 1.0 79.9
psbZ-trnG-GCC_p2 GCTTCTCCTGATGGTTGGTC CGTCTTCTCCTTGGCAAAGA 55.0 50.0 58.3 57.8 484-493 bp 1.0 79.5
psbZ-trnG-GCC_p3 TTGCTTCTCCTGATGGTTGG ATGACTTCATTTTGGGCGGA 50.0 45.0 57.8 57.8 524-533 bp 1.0 79.4
psbZ-trnG-GCC_p4 TTGCTTCTCCTGATGGTTGG TGACTTCATTTTGGGCGGAT 50.0 45.0 57.8 57.8 523-532 bp 1.0 79.4
psbZ-trnG-GCC_p5 GCTTCTCCTGATGGTTGGTC TGACTTCATTTTGGGCGGAT 55.0 45.0 58.3 57.8 521-530 bp 1.0 79.0