Hotspot

trnE-UUC-rpoB

LSC · 12186-13110 · 925 bp

Back to Dahlia

Pi 0.0087
MarkerSeek score 64.30
Primer available yes
Label trnE-UUC-rpoB

Feature Metadata

label_name
trnE-UUC-rpoB
feature_type
igs
region
LSC
length_bp
925
variable_sites
8
pi
0.0087
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-rpoB_p1 GGGACTTGAACCCTCGTTTT CTTCTGCCAAACCCTGATCA 50.0 50.0 58.0 57.8 1059-1063 bp 1.0 79.2
trnE-UUC-rpoB_p2 TAACCACTGGACCATAGGGG CTTCTGCCAAACCCTGATCA 55.0 50.0 58.1 57.8 1018-1022 bp 1.0 78.5
trnE-UUC-rpoB_p3 CCCTCGTTTTCTCCGTGAAA CTTCTGCCAAACCCTGATCA 50.0 50.0 58.1 57.8 1049-1053 bp 1.0 78.3
trnE-UUC-rpoB_p4 TTGAACCCTCGTTTTCTCCG CTTCTGCCAAACCCTGATCA 50.0 50.0 58.1 57.8 1054-1058 bp 1.0 78.3
trnE-UUC-rpoB_p5 GGGACTTGAACCCTCGTTTT TCTTCTGCCAAACCCTGATC 50.0 50.0 58.0 57.5 1060-1064 bp 1.0 76.8