Hotspot

psbM-trnD-GUC

LSC · 11023-11663 · 641 bp

Back to Dahlia

Pi 0.0078
MarkerSeek score 63.20
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
641
variable_sites
5
pi
0.0078
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 GTTTGACTGGCTGTTTTTACGT TTCAATAGGCAAGAGCACCG 40.9 50.0 58.0 58.3 803-811 bp 1.0 62.2
psbM-trnD-GUC_p2 TTGACTGGCTGTTTTTACGT TTCAATAGGCAAGAGCACCG 40.0 50.0 55.8 58.3 801-809 bp 1.0 60.9
psbM-trnD-GUC_p3 AGTTTGACTGGCTGTTTTTACG TTCAATAGGCAAGAGCACCG 40.9 50.0 57.7 58.3 804-812 bp 1.0 60.1
psbM-trnD-GUC_p4 TCAGTTTGACTGGCTGTTTT TTCAATAGGCAAGAGCACCG 40.0 50.0 55.7 58.3 806-814 bp 1.0 60.0
psbM-trnD-GUC_p5 TTTGACTGGCTGTTTTTACGT TTCAATAGGCAAGAGCACCG 38.1 50.0 56.6 58.3 802-810 bp 1.0 59.1