Hotspot

ndhF-rpl32

SSC · 111972-112715 · 744 bp

Back to Dactylorhiza

Pi 0.0188

MarkerSeek score 54.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 744
variable_sites: 23
pi: 0.0188
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAGCGGAAAAAGGGGTATT	TCCACCGGACAGTCAAAAAG	50.0	50.0	57.9	58.0	831-863 bp	1.0	84.0
ndhF-rpl32_p2	ACATAGTAACTGGGAGCGGA	TCCACCGGACAGTCAAAAAG	50.0	50.0	57.8	58.0	843-875 bp	1.0	83.8
ndhF-rpl32_p3	CATTCCCACGAGTTCCCAAA	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.1	58.0	1250-1282 bp	1.0	82.6
ndhF-rpl32_p4	AACACATTCCCACGAGTTCC	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.1	58.0	1254-1286 bp	1.0	82.5
ndhF-rpl32_p5	CGGATTTAGCAATTGCACCG	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.2	58.0	1506-1538 bp	1.0	81.4