Hotspot

rpl36-infA

LSC · 78844-78959 · 116 bp

Back to Dactylorhiza

Pi 0.0629

MarkerSeek score 80.00

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 116
variable_sites: 13
pi: 0.0629
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	CCTACGAATCAGGCGACATT	TGATTCAACCAGGGGACGTA	50.0	50.0	57.8	58.4	231-235 bp	1.0	79.9
rpl36-infA_p2	CCTACGAATCAGGCGACATT	CCGGCGCAGTTTTATACGTA	50.0	50.0	57.8	58.4	291-295 bp	1.0	78.2
rpl36-infA_p3	CCTACGAATCAGGCGACATT	ATCCGGCGCAGTTTTATACG	50.0	50.0	57.8	58.8	293-297 bp	1.0	75.5
rpl36-infA_p4	CCTACGAATCAGGCGACATT	GATTCAACCAGGGGACGTAT	50.0	50.0	57.8	57.0	230-234 bp	1.0	74.7
rpl36-infA_p5	CCTACGAATCAGGCGACATT	TATGATTCAACCAGGGGACG	50.0	50.0	57.8	57.0	233-237 bp	1.0	74.6