Hotspot

psbB-psbT

LSC · 72777-73166 · 390 bp

Back to Dactylorhiza

Pi 0.0179

MarkerSeek score 57.60

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 390
variable_sites: 16
pi: 0.0179
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GTGCCAGAACCTTGTTCAGA	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	58.0	57.6	599-654 bp	1.0	71.8
psbB-psbT_p2	TGCCAGAACCTTGTTCAGAG	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.7	57.6	598-653 bp	1.0	70.0
psbB-psbT_p3	TCAGAGATGTTTTTGCCGGT	TGGAACTTTAGGTGGTTCTCG	45.0	47.6	57.7	57.6	584-639 bp	1.0	70.0
psbB-psbT_p4	GTGCCAGAACCTTGTTCAGA	GGAACTTTAGGTGGTTCTCGA	50.0	47.6	58.0	57.3	598-653 bp	1.0	69.6
psbB-psbT_p5	TGCCAGAACCTTGTTCAGAG	GGAACTTTAGGTGGTTCTCGA	50.0	47.6	57.7	57.3	597-652 bp	1.0	67.8