Hotspot

psaJ-rpl33

LSC · 65346-65907 · 562 bp

Back to Dactylorhiza

Pi 0.0338

MarkerSeek score 72.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 562
variable_sites: 35
pi: 0.0338
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTGGGTCTTTAGCGGGTCTA	GGTCAACCTATTCACCCGTC	50.0	55.0	58.1	58.0	1092-1214 bp	1.0	86.2
psaJ-rpl33_p2	ACCCGTGACATTTTGTACCC	GGTCAACCTATTCACCCGTC	50.0	55.0	58.1	58.0	1575-1696 bp	1.0	85.5
psaJ-rpl33_p3	CCCGTGACATTTTGTACCCA	GGTCAACCTATTCACCCGTC	50.0	55.0	58.1	58.0	1574-1695 bp	1.0	85.5
psaJ-rpl33_p4	ACAGGATTTGAACCCGTGAC	GGTCAACCTATTCACCCGTC	50.0	55.0	58.1	58.0	1586-1707 bp	1.0	85.4
psaJ-rpl33_p5	CAGGATTTGAACCCGTGACA	GGTCAACCTATTCACCCGTC	50.0	55.0	58.1	58.0	1585-1706 bp	1.0	85.4