Hotspot

trnF-GAA-ndhJ

LSC · 47232-47770 · 539 bp

Back to Dactylorhiza

Pi 0.0438

MarkerSeek score 77.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 539
variable_sites: 45
pi: 0.0438
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	481-679 bp	1.0	88.3
trnF-GAA-ndhJ_p2	GCTAGGATGATGCACGGAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.0	57.9	543-741 bp	1.0	87.9
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	471-669 bp	1.0	87.8
trnF-GAA-ndhJ_p4	GCTAGGATGATGCACGGAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.0	58.2	533-731 bp	1.0	87.3
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	523-721 bp	1.0	86.7